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When CO2 gas infused into the skin, immediately diffuses into the cutaneous and muscular microcirculatory levels, resulting in higher tissue oxygenation and neoangiogenesis. Applying carbon dioxide topically creates an acidic environment in the skin. When infused into the skin, CO2 immediately diffuses at the cutaneous and muscular microcirculatory levels. This triggers the body’s response to a decrease in the affinity of hemoglobin for oxygen due to an increase in CO2 and increased oxygen delivery to the treated area, along with vascular endothelial growth factors.